Figure 2

Effect of enforcing SSX2IP on peritoneal spreading and liver metastasis. 1 × 10⁶ cells per mouse were injected into peritoneal cavity or caudal vena. A. BEL-7402, BEL-7402^Vector and BEL-7402^SSX2IP, the three cell lines, were injected into the peritoneal cavity of nude mice. 6 weeks later, we took the photographs of the peritoneal nodles. B. The peritoneal nodles were significantly enforced in SSX2IP overexpression group, compared with the parental cells and empty vector-transfected cells groups(6.2 ± 0.84 vs 2.6 ± 0.89, 2.4 ± 1.14, **P < 0.001). C. BEL-7402, BEL-7402^Vector and BEL-7402^SSX2IP, the three cell lines, were injected into the caudal vena of nude mice. 10 weeks later, we took the photographs of the nude mice liver. D. Statistical proportion of liver metastasis mice. In SSX2IP overexpression group, 7 out of 10 mice show tumorigenicity in liver, which is significantly higher than that in parental and empty vector-transfected groups (2/10 and 1/10, P = 0.023).