Figure 2

Response of Fz1 expression to exogenous Wnt3a ligand in normal colon-derived NCM460 cells. Panel A: mRNA levels by quantitative real-time PCR without and with 24 h of exposure to Wnt3a. p<0.005 for the difference (3 separate experiments, all in triplicate). Panels B, C: Fluorescent immunohistochemistry of NCM460 cells in tissue culture without (B) and with (C) 24 h of exposure to Wnt3a. Panel D: Quantified light intensity from immunofluorescence of anti-Fz1 antibody staining in NCM460 cells without and with 24 h of exposure to Wnt3a. p<0.001 for the difference (3 independent experiments with 25 individual fields recorded for each).