Figure 1
Expression of frizzled receptors by fluorescent immunohistochemistry in patient derived samples. Panels A, B, C: Fz1 expression in colon cancer – DAPI (A), anti-Fz1 antibody (B), merged images (C) – showing uniform expression. Panels D, E, F: Fz1 expression in normal colonic mucosa distant (>2 cm) from colon cancer – DAPI (D), anti-Fz1 antibody (E), merged images (F) – showing lack of significant expression. Panels G, H, I: Fz1 expression in normal colonic mucosa adjacent to colon cancer – DAPI (G), anti-Fz1 antibody (H), merged images (I) – showing significant Fz1 expression. Panels J, K, L: Fz2 expression in colon cancer – DAPI (J), anti-Fz2 antibody (K), merged images (L) – showing uniform expression. Panels M, N, O: Fz2 expression in normal colonic mucosa distant (>2 cm) from colon cancer – DAPI (M), anti-Fz2 antibody (N), merged images (O) – showing uniform expression. Panel P: Composite of multiple merged images of Fz1 expression in colon cancer and normal colonic mucosa adjacent to tumor showing expression in both the cancer and in the adjacent normal mucosa. Scale bar = 50 um. Panel Q: DAB immunohistochemistry with anti-Fz1/(2) antibody of colon cancer, normal colonic mucosa adjacent to the tumor (arrows) and normal colonic mucosa distant from the tumor (insert) showing significant expression in the cancer and in the adjacent normal colonic mucosa. Panel R: Higher magnification from image displayed in Panel Q of normal colonic mucosa adjacent to colon cancer showing expression. Panel S: Higher magnification from image displayed in Panel Q of normal colonic mucosa distant from colon cancer showing lack of expression.