Figure 5
From: Anti-KIT designer T cells for the treatment of gastrointestinal stromal tumor
In vivo testing of human anti-KIT designer T cells. We used a subcutaneous xenograft model whereby GIST 882 cells were injected subcutaneously into immunodeficient mice prior to treatment with human anti-KIT dTc. Median results from a single representative experiment (A) and pooled data from 3 experiments (B) are shown. Activated CIR- T cells were used as negative controls. (C) Median tumor sizes at Day 5, Day 10, and Day 15 post-infusion are indicated by horizontal bars along with individual tumor measurements represented by the data points. (D) We performed immunohistochemistry (top row 10x, bottom row 40x) to detect tumor infiltrating CD3+ T cells (arrows). (E) Routine H&E was used to assess the degree of tumor necrosis (asterisks) in mice treated with 1st or 2nd gen dTc, both with and without supplemental IL2 (left column 10x, right column 40x).