Figure 2

Frequencies of CD27⁺CD28⁺and CD4⁺FoxP3⁺T cells in IL-2, IL-15, IL-21 or no cytokine aAPC-expanded TIL. NSCLC/OvCa/mel-derived TIL were expanded by stimulation with IL-2, IL-15, IL-21 or no cytokine producing aAPC and used for further phenotypic and functional analysis. Percentage of CD27⁺CD28⁺ T cells was determined after two stimulations, and percentage of CD4⁺FoxP3⁺ T cells was determined after one or two stimulations by flow cytometry. Percentages of CD27⁺CD28⁺ is given for 13 NSCLC/OvCa/mel patients within A) the CD3⁺CD8⁺ T cell population and B) the CD3⁺/CD4⁺ T cell population. Percentage of suppressive FoxP3⁺ T cells is given for 13 patients C) as percentage of FoxP3 within CD4⁺ T cell population or D) as percentage of CD4⁺FoxP3⁺ cells within the total TIL population. Differences were compared using repeated measures ANOVA’s with a Tukey post test when data followed Gaussian distribution and by using a Friedman test with a Dunn’s post test when data did not pass the normality test. Differences were considered significant when p < 0.05, as indicated with an asterisk (* p < 0.05, ** p < 0.01), *** p < 0.001).