Figure 3

Increased accumulation of MDSC is associated with increased levels of lipid mediators and ARG1. A) Shows accumulation of COX2⁺ cells in co-cultures containing MiaE or MiaM cells in the presence or absence of aATC or aATC+Th₁ cytokines. Bottom panel show a correlation between COX2 positive cells and frequency of monocytic and granulocytic MDSC in co-cultures for both cell lines. B) Shows PGE₂ levels in the supernatants from co-cultures containing MiaM cells in the presence or absence of aATC or aATC+Th₁ cytokines. Bottom panel show a strong correlation between PGE₂ levels and frequency of monocytic and granulocytic MDSC in co-cultures for MiaM cells. C) Shows presence of ARG1⁺ cells in co-cultures containing MiaE or MiaM cells in the presence or absence of aATC or aATC+Th₁ cytokines. Bottom panel show a correlation between of ARG1 positive cells and frequency of monocytic and granulocytic MDSC in co-cultures for both cell lines. */**/*** signifies statistically significant differences (*P < 0.05, **P < 0.01, ***p<0.001).