Figure 2

aATC can attenuate the suppressive properties of MDSC. CD33⁺ cells isolated either from control condition or from co-cultures containing aATC were added to cytotoxicity assay, proliferation assay and anti-CD3 stimulated T cells for 24-72 h. A) Shows the suppressive effect of CD33⁺ MDSC on anti-CD3-stimulated autologous T-cell proliferation. Proliferation was significantly suppressed by more than 50% in the presence of CD33⁺ MDSC isolated from control co-cultures, and this suppression was reversed if CD33⁺ MDSC were isolated from aATC containing co-cultures. B) Shows the suppressive effect of CD33⁺ MDSC on aATC mediated cytotoxicity. C) Top panel, right histogram shows CD71 expression on stimulated CD3⁺ T cells (positive control); middle histogram shows suppressive effect of CD33⁺ MDSC isolated from control co-cultures (without aATC) and right histogram show attenuated suppressive effect on CD71 expression when CD33⁺ cells were isolated from co-cultures that contained aATC. Second panel, right histogram shows NKG2D expression on stimulated CD3⁺ NK T cells (positive control); middle histogram shows the suppressive effect of CD33⁺ MDSC isolated from control co-cultures (without aATC) and right histogram shows the attenuated suppressive effect on NKG2D expression when CD33⁺ cells were isolated from co-cultures that contained aATC on anti-CD3-stimulated T-cells. Third panel, right histogram shows IFN-γ positive T cells upon stimulation with PC cells (positive control); middle histogram show suppressive effect of CD33⁺ MDSC isolated from control co-cultures (without aATC) on IFN-γ production and right histogram show attenuated suppressive effect on IFN-γ production when CD33⁺ cells isolated were from co-cultures that contained aATC. Bottom panel, right histogram shows CD62L expression (migration marker) on naive T cells (positive control); middle histogram show suppressive effect of CD33⁺ MDSC isolated from control co-cultures (without aATC) and right histogram show attenuated suppressive effect on CD62L expression when CD33⁺ cells added were isolated from co-cultures that contained aATC.