Figure 4

Overexpression of miR-655 decreases the expression of MMP2, MMP9 and PTTG1 in EC9706 and KYSE150 cells. (a) Western blot analysis of PTTG1 expression in transfected cells. β-actin was used as a reference. Compared to NC and Blank groups, the expression levels of PTTG-1, MMP2 and MMP9 in miR-655 group were reduced. (b) Cells of three groups were concentrated to assess MMP-2 and MMP-9 secretion using ELISA. miR-655 group exhibited an evidently lowered amount of MMP2 and MMP9 compared with NC and Blank groups (P < 0.05). Blank, non-transfected cells; NC, cells transfected with scrambled miR-655 negative control; miR-655, cells transfected with miR-655 mimics.