Figure 6

Unaltered expression patterns of the NCRs NKp30, NKp44 and NKp46 on enriched NKs from the patient at diagnosis, in remission and the healthy twin. (A) Expression pattern of NCRs on enriched NKs from the patient in remission (red) and the healthy twin (blue) compared with cells stained with an isotype control mAb (black). (B) To analyze NCR expression on enriched NKs from the patient at diagnosis, an additional sorting step for CD16 positive cells was performed to focus the analysis on the small subpopulation of NKs. NKs from the patient at diagnosis (red) are compared with cells stained with an isotype control mAb (black). (C) Gates chosen to generate data shown in panels A) and B). Left: gate chosen to identify MACS-sorted cells (negative selection for NK cells). Right: additional gate used for identification of the CD16 pos subset (lower right quadrant) of the MACS sorted cells from the patient sample obtained at diagnosis (Methods).