Figure 6

Cell aggregation assay and cell adhesion assay. A:The formation of cell colony was obviously inhibited in shCDH12 group compared with control groups. B: Cell numbers adhered to HUVEC monolayer in shCDH12 group is less than negative control group after PBS elution which demonstrated the function of CDH12 in tumor cell and endothelial cell junction. As the transfectant (shRNA and scrambled shRNA) is marked with GFP, so the cells adhered to the monolayer was showed as green dots. C: The numbers of the cells were counted in five random views and the statistical result is presented as histogram (*P<0.05).