Effects of SelS on the expression of PKCα in ECs stimulated with or without H
. A. The level of PKCα gene expression presented as a ratio to GAPDH following real-time PCR analysis. B. The level of PKCα protein expression presented as a ratio to β-actin following densitometric analysis of western blots. C. Western blot analysis of PKCα expression in HUVECs not stimulated with H2O2. D. Western blot analysis of PKCα expression in HUVECs stimulated with 800 μmol/L H2O2. White column: no H2O2 stimulation; shaded column: stimulation with 800 μmol/L H2O2. 1, 2, 3, 4: control group, pc-SelS group, vector control group and siRNA-SelS group, respectively. *P < 0.01 compared with the vector control group. n = 3.