Figure 5

Effects of SelS on the expression of PKCα in ECs stimulated with or without H ₂ O ₂ . A. The level of PKCα gene expression presented as a ratio to GAPDH following real-time PCR analysis. B. The level of PKCα protein expression presented as a ratio to β-actin following densitometric analysis of western blots. C. Western blot analysis of PKCα expression in HUVECs not stimulated with H₂O₂. D. Western blot analysis of PKCα expression in HUVECs stimulated with 800 μmol/L H₂O₂. White column: no H₂O₂ stimulation; shaded column: stimulation with 800 μmol/L H₂O₂. 1, 2, 3, 4: control group, pc-SelS group, vector control group and siRNA-SelS group, respectively. *P < 0.01 compared with the vector control group. n = 3.