Figure 2

The distribution of OCC within AMS is following expression of MSC markers. A-D. Confocal microscopy tile scan reconstruction of Skov3_eGFP (green) distribution after staining with CD90 (red) and CD73 (yellow). The distribution of OCC (B) is following the distribution of CD90 (C) and CD 73 (D). Using 3D z-stack reconstruction of the tumor, we could measure the tumor depth infiltration of OCC within AMS and the relations between OCC and surrounding CD73+ amniotic stromal cells (B, arrows). E-F. Confocal tile scan reconstruction showing the distribution of OCC within AMS in regions rich in MSC (E) and without MSC (F), with their related depth of infiltration. (G). OCC infiltration was significantly increased in regions rich in MSC (*p<0.05).