Table 1 MACS technical data from primary expanded chondrocytes or disc cells (“P0 cells”)

chondrocytes

Cho2

8.9

1.6

4.5

68.0

18.0

n.d.

n.d.

n.d.

Cho4

16.2

4.0

5.0

55.7

24.8

95.9

53.8

55.2

Cho5

8.8

2.2

4.7

78.4

24.5

86.6

47.8

74.7

Cho6

10.0

3.2

4.3

75.0

32.0

87.4

26.2

51.2

Cho7

37.2

4.8

30.5

94.9

12.9

75.4

12.0

24.9

Cho8

8.8

1.3

3.3

52.2

14.9

90.6

28.0

42.8

Cho9

12.8

2.9

6.2

71.1

22.7

78.5

24.9

41.7

Cho10

24.1

6.8

9.2

66.3

28.1

92.8

38.4

63.0

Cho11

13.1

2.0

6.5

64.7

14.9

94.5

30.1

43.7

Cho12

8.4

0.5

4.7

62.1

6.0

70.2

37.4

31.1

Cho13

19.3

4.4

8.7

68.0

22.7

97.0

52.7

74.6

Cho14

21.9

6.7

5.5

55.7

30.6

97.2

69.2

89.3

Cho15

9.0

0.9

3.7

51.4

10.4

95.1

32.2

42.2

mean

15.3

3.2

7.4

66.4

20.2

88.4

37.7

52.9

sd

8.5

2.1

7.2

12.0

8.0

9.1

15.7

19.2

disc cells

Disc1

n.d.

0.6

6.4

n.d.

n.d.

n.d.

n.d.

n.d.

Disc2

7.6

1.2

1.6

37.0

16.1

n.d.

n.d.

n.d.

Disc3

13.7

1.5

4.9

46.3

10.6

98.1

45.1

49.4

mean

10.7

1.1

4.3

41.6

13.3

98.1

45.1

49.4

sd

4.3

0.4

2.5

6.6

3.9

W8B2 sorting

Cell numbers [ x 10 ⁶ ]

Analytical flow cytometry: % W8B2 positive cells in

Cell type

Initial cells

Bound fraction

Flowthrough fraction

Total recovery [%]

% Bound cells from initial cells

Bound fraction

Flowthrough fraction

Unsorted fraction

chondrocytes

Cho3

9.5

0.6

5.8

67.3

5.8

n.d.

n.d.

n.d.

Cho4

16.2

0.7

7.2

48.5

4.3

72.8

8.7

5.6

Cho5

8.8

0.7

6.3

79.4

8.2

63.0

11.9

16.8

Cho14

22.4

1.1

7.3

37.4

4.8

98.8

34.5

50.4

mean

14.2

0.8

6.6

58.1

5.8

78.2

18.4

24.3

sd

6.4

0.2

0.7

18.8

1.7

18.5

14.1

23.3