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Table 1 MACS technical data from primary expanded chondrocytes or disc cells (“P0 cells”)

From: Maintenance of “stem cell” features of cartilage cell sub-populations during in vitro propagation

W5C5 sorting
  Cell numbers [ x 106]    Analytical flow cytometry: % W5C5 positive cells in
Cell type Initial cells Bound fraction Flowthrough fraction Total recovery [%] % Bound cells from initial cells Bound fraction Flowthrough fraction Unsorted fraction
chondrocytes         
Cho2 8.9 1.6 4.5 68.0 18.0 n.d. n.d. n.d.
Cho4 16.2 4.0 5.0 55.7 24.8 95.9 53.8 55.2
Cho5 8.8 2.2 4.7 78.4 24.5 86.6 47.8 74.7
Cho6 10.0 3.2 4.3 75.0 32.0 87.4 26.2 51.2
Cho7 37.2 4.8 30.5 94.9 12.9 75.4 12.0 24.9
Cho8 8.8 1.3 3.3 52.2 14.9 90.6 28.0 42.8
Cho9 12.8 2.9 6.2 71.1 22.7 78.5 24.9 41.7
Cho10 24.1 6.8 9.2 66.3 28.1 92.8 38.4 63.0
Cho11 13.1 2.0 6.5 64.7 14.9 94.5 30.1 43.7
Cho12 8.4 0.5 4.7 62.1 6.0 70.2 37.4 31.1
Cho13 19.3 4.4 8.7 68.0 22.7 97.0 52.7 74.6
Cho14 21.9 6.7 5.5 55.7 30.6 97.2 69.2 89.3
Cho15 9.0 0.9 3.7 51.4 10.4 95.1 32.2 42.2
mean 15.3 3.2 7.4 66.4 20.2 88.4 37.7 52.9
sd 8.5 2.1 7.2 12.0 8.0 9.1 15.7 19.2
disc cells         
Disc1 n.d. 0.6 6.4 n.d. n.d. n.d. n.d. n.d.
Disc2 7.6 1.2 1.6 37.0 16.1 n.d. n.d. n.d.
Disc3 13.7 1.5 4.9 46.3 10.6 98.1 45.1 49.4
mean 10.7 1.1 4.3 41.6 13.3 98.1 45.1 49.4
sd 4.3 0.4 2.5 6.6 3.9    
W8B2 sorting
  Cell numbers [ x 10 6 ]    Analytical flow cytometry: % W8B2 positive cells in
Cell type Initial cells Bound fraction Flowthrough fraction Total recovery [%] % Bound cells from initial cells Bound fraction Flowthrough fraction Unsorted fraction
chondrocytes         
Cho3 9.5 0.6 5.8 67.3 5.8 n.d. n.d. n.d.
Cho4 16.2 0.7 7.2 48.5 4.3 72.8 8.7 5.6
Cho5 8.8 0.7 6.3 79.4 8.2 63.0 11.9 16.8
Cho14 22.4 1.1 7.3 37.4 4.8 98.8 34.5 50.4
mean 14.2 0.8 6.6 58.1 5.8 78.2 18.4 24.3
sd 6.4 0.2 0.7 18.8 1.7 18.5 14.1 23.3