Figure 3

A Effect of ISBn alone or in combinations on the distribution of growing Caco-2 cell populations. Data represent the percentage of cells in each phase of the cell cycle. Cell cycle distribution was determined by DNA flow cytometric analysis. Samples from exponentially growing Caco-2 cells were analysed after 24 h of treatment with 100 and 240 μM of resveratrol and polydatin alone or in combinations. Numbers indicate percentage of cells in G0/G1, S and G2/M phases. Data are representative of four separate analyses. In the inset of panel A was reported the western blot analysis of p21 protein and tubulin expression in growing. B: Effect of ISBn on of Caco-2 cells differentiation. The differentiation of Caco-2 cells assessed by measurement of ALP activity after 24 h of culture with 0, 240, and 100 μM of Pol, Res and their combination. Summary data shown are means ± SEM; (n = 4; *P < 0.05).assessed by measurement of ALP activity.