Figure 3

dl 1520 promoted endostatin expression by Ad-Endo in GC cells. GC cells were infected with Ad-Endo alone or combination with dl 1520, and then the culture supernatants were collected at different time points. The endostatin concentrations were measured using a human endostatin ELISA kit (Shanghai ExCell Biology, Inc., Shanghai, China). The minimum detectable dose using this kit is 30 pg/mL. A) Endostatin concentrations in the culture supernatants of GC cells at different time points after infection with 10 MOIs of Ad-Endo alone or in combination with 10 MOIs of dl 1520 (two-way ANOVA, *p<0.05, **p<0.01 compared to Ad-Endo alone). B) Endostatin concentrations in the culture supernatants at 48 hours after infection with 10 MOIs of Ad-Endo alone or in combination with increasing MOIs of dl 1520 (one-way ANOVA, *p<0.05, **p<0.01 compared to that of dl 1520 at 0 MOIs).