Myocardial IRI is mitigated by activated Notch1 signaling. A, Myocardial cell viability was enhanced by ectopic expression of N1ICD and inhibited by knockdown of N1ICD. B, IRI induced apoptosis was inhibited by ectopic expression of N1ICD but promoted by knockdown of N1ICD. The apoptosis of H9c2 cells was detected based on Annexin V/FITC and PI staining and flow cytometry. Data were presented as mean±SD, n=3. Mock1 indicated cells infected with empty vector, Mock2 indicated cells infected with vector containing nonspecfic sequences. ▲P>0.05, *P<0.05, **P<0.01.