Figure 1

Identification of LV-N1ICD and LV-N1ICD-shRNA vectors. A, Western blots showing the expression of Flag tagged N1ICD in 293T cells as indicated. B, Western blots showing the expression of Flag tagged N1ICD in H9c2 cells as indicated. C, Western blots showing the expression of Flag tagged N1ICD in the hearts of the rats as indicated, D, Western blots showing the expression of Flag tagged N1ICD in 293T cells transfected with pGC-FU-N1ICD-3Flag along with negative control pGVC112-shRNA (Mock), pGVC112-N1ICD-shRNA-1 (N1ICD-shRNA-1), pGVC112-N1ICD-shRNA-2 (N1ICD-shRNA-2), pGVC112-N1ICD-shRNA-3 (N1ICD-shRNA-3), or pGVC112-N1ICD-shRNA-4 (N1ICD-shRNA-4). E, Western blots showing the expression of endogenous N1ICD in H9c2 cells infected by lentivirus as indicated, F, Western blots showing the expression of endogenous N1ICD in the hearts of the rats infected by lentivirus as indicated. β-actin was loading control.