Figure 3

Effect of Cetuximab pretreatment on phagocytosis of IGROV-1 cells. IGROV-1 target cells were stained green with PKH67 (A, right lower quadrant) and RAW264.7 effector cells were stained red with PKH26 (B, left upper quadrant). Tumor targets were pre-incubated for 72 h with 5 μg/ml Cetuximab (F,G,H) or left untreated (C,D,E). At the end of treatment, target and effector cells were mixed at effector/target (E/T) ratio of 3:1 in complete medium and incubated for an additional 12 h in overload conditions of monoclonal antibody (10 μg/ml). The percentage of double-positive cells present in the upper right quadrant (quadrant Q2) of the dot plots represents the percentage of RAW264.7 cells phagocytosing green-stained tumor cells. Data were obtained in triplicate and are representative of one of three experiments with similar results.