Figure 3

Effect of BBR on H/R-induced apoptosis in HK-2 cells. (A) Under the inverted phase contrast microscope (×100), control and BBR groups showed the normal elliptical morphology. H/R caused extensive blebbing morphology and a decreased cell population, while BBR prevented the injury. (B) Under fluorescence microscope (×400), apoptotic cells stained with Hechst33258 showed nucleic fragmentation with dense chromatin. They were the typical morpyological changes of apoptosis. (C) Quantitative assessment of apoptotic cells by annexin V-FITC/PI staining. Intact cells were V-/PI-, early apoptotic cells were V+/PI- , late apoptotic cells were V+/PI+ and necrotic cells were V-/PI+. (D) Flow cytometry results were showed as quantitative bar graphs. The results were expressed as mean ±S.D. (n=5). ^*p<0.05 vs. control, ^#p<0.05 vs. H/R group without BBR treatment.