SPAR values of 6 ion pairs from peptide m/z 520.3. Box plot figure shows values of 4-6 ion pairs generated from peptide m/z 520.3 in 86 serum samples from CHB patients. A: Patients were classified in 5 groups according to fibrosis stages (S0: n = 13, S1: n = 22, S2: n = 20, S3: n = 16, S4: n = 15). SPAR values of 6 ions of m/z 520.3 display a statistically significant decrease in groups S1 ~ S4 as compared to S0. Different colors represent different ions (blue = 520.3/176.1, green = 520.3/353.7, brown = 520.3/459.8, purple = 520.3/503.3, yellow = 520.3/351.3, red = 520.3/593.1). B: SPAR values decrease along with liver inflammation severity in the 5 groups classified by inflammation grades. C: SPAR values have no statistically significant difference between groups classified by HBeAg carrier status. D: SPAR values have no statistically significant difference among groups classified by HBV-DNA levels (HBV-DNA: 104 ~ 105; 105 ~ 106; 106 ~ 107; ≥107).