Figure 1

Unsupervised clustering analysis of miRNA expression. The heat map illustrates the result of the two-way hierarchical clustering of miRNAs and samples. Each row represents one miRNA and each column represents one sample, including pre-treatment, post-Temsirolimus alone, or post-combination treatment samples from patients #1 through 12. Samples resubmitted for quality assurance purposes are marked by a shaded oval enclosing the duplicate samples. The color scale illustrates the relative expression level (log median ratio, LMR) of a miRNA across all samples: red color represents an expression level above the mean, blue color represents expression lower than the mean. Clustering was performed on all samples and on the 50 miRNAs with the highest standard deviation across the sample set.