Expression of SATB1 mRNA and protein in various types of NPC and immortalized NP-69 cell lines. (A): Well-differentiated CNE1 cells, poorly- differentiated CNE2Z cells, undifferentiated C666-1 cells and the immortalized non-cancerous NP-69 cells were seeded in 24-well plates (n = 3) and harvested for analysis of SATB1 expression by quantitative reverse transcription polymerase chain reaction (qRT-PCR). β-actin was used as a loading control. ** P <0.01 vs NP-69, △P <0.05 vs CNE1, # P <0.05 vs CNE2Z. (B): Western blotting was used to detected SATB1 protein expression in CNE1, CNE2Z, C666-1 and NP-69 cell lines. β-actin was used as a loading control.