Figure 4

Morphometric and biochemical analyses in rAAV-transduced chondrocytes in situ . Explants were transduced with rAAV-lacZ or rAAV-hTGF-β as described in Figure 1 and maintained in culture for up to 90 days. The samples were then fixed and histologically processed at the denoted time points to monitor the % of BrdU labeling (A), the DNA contents (B), cells densities (C), % of apoptotic cells (D), proteoglycan contents (E), % of matrix staining (F), type-II collagen contents (G), % of type-II collagen immunostaining (H), type-X collagen contents (I), and % of type-X collagen immunostaining (J).