Figure 1

Detection of TGF-β expression in rAAV-transduced human normal and OA chondrocytes in vitro and in situ. Cells (A) and explants (B) were transduced by direct administration of the vectors (rAAV-lacZ or rAAV-hTGF-β: 40 μl each vector) and maintained in culture for 21 days in vitro and for 90 days in situ. The samples were then fixed and histologically processed to monitor the expression of TGF-β by immunocyto-/-histochemical detection (A: magnification x4; B: magnification x20, view of the middle zone).