Figure 1
From: Upregulation of M3 muscarinic receptor inhibits cardiac hypertrophy induced by angiotensin II
Upregulation of M 3 -mAChR expression in in vitro and in vivo models of hypertrophy. (a, b) Western blot analysis of M3-mAChR and M2-mAChR protein levels in ventricular tissues from mice with chronic angiotensin II (Ang II) (0.6 mg/kg per day) infusion for 14 days (n = 4 mice per group); (c) Western blot analysis of M3-mAChR protein level in neonatal rat ventricular myocytes (NRVMs) incubated with Ang II (0.1 μM) for 48 hours (n = 3); (d, e) Western blot analysis of M3-mAChR and M2-mAChR protein levels in ventricular tissues from mice with pressure overload by transverse aotic constriction (TAC) for 14 days (n = 4 mice per group); (f) M3-mAChR protein level from NRVMs incubated with isoproterenol (ISO) (10 μM) for 48 hours (n = 3). GAPDH served as loading control. Values were expressed as mean ± SEM and normalized to the CTRL group. *P < 0.05 vs. CTRL group.