Figure 1
Tumor growth inhibition by intratumor injection of UV-Tianjin. A to C, 5 × 106 CT26 cells were inoculated into subcutaneous space on the back of BALB/c mice. Particles of UV-Tianjin (5 × 108 each) or saline were injected into tumors on days 4, 8, and 12. A, UV-Tianjin-treated or saline-treated BALB/c mice 3 weeks after tumor inoculation. The tumors treated by UV-Tianjin were much smaller than those treated by saline. B, The growth of UV-Tianjin-treated tumors was strongly suppressed compared with saline-treated tumors. Each point in the curve was expressed as the mean ± SD of the relative tumor volume Vt/V0 (where Vt denotes the tumor volume at test time point and V0 denotes the corresponding initial tumor volume at the beginning of treatment). *P < 0.01 vs. control group. Arrows indicate the timing of injection. C, Survival curve of the mice bearing CT26 colon carcinoma following three consecutive injections of UV-Tianjin or saline, revealed a significant increasing of survival rate from 30% to 100% at day 60 by UV-Tianjin. *P < 0.01 vs. control group. D, Microscopic view of CT26 cells infected with live Tianjin strain or UV-Tianjin on 4 dpi (days post infection). a, Live Tianjin strain infection produced an obvious cytopathic effect (CPE) characterized by cell rounding and detachment from the monolayer. b, UV-Tianjin infection can’t produce visible CPE. c, Healthy control. Photographs are shown at 100× magnification. Results are representative of three independent experiments.