Figure 4

Docetaxel resistant cell line shows elevated FOXM1 mRNA and protein expression levels. (A) Cells in different cycle of molecular evolution assay were treated with docetaxel respectively, and their rates of cell viability were measured by MTT assay. R0 represents the untreated control cell line, whereas R2, R4, R6 and R8 represents AGS cells that are treated with 0.02 mg/L docetaxel for two, four, six and eight times, separately. IC50 for cells in R0, R2, R4, R6 and R8 was 0.026, 0.033, 0.054, 0.098, 0.190 mg/L. (B) FOXM1 mRNA transcript levels in R0, R2, R4, R6 and R8 cells were determined by RT-PCR analysis. (C) Western blot analysis was performed to detect the relative protein expression levels of FOXM1 in the different treated round. Statistical analysis was performed using Student’s t tests. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 significant.