Figure 3
Antigen-specific cytolysis of anti-FAP-F19-∆CD28/CD3 ζ re-directed T cells. Re-directed T cells recognizing FAP (anti-FAP-F19-∆CD28/CD3ζ (square) and NY-ESO-1157-165 peptide in the HLA-A*02:01 context (anti-NY-ESO-1-T1-∆CD28/CD3ζ) (circle) were cultured with MSTO-211H cells (a, FAP positive), HT1080FAP-luc cells (b, FAP positive), primary fibroblasts (c, FAP positive), HT1080PA-luc cells (d, FAP negative) and T2-1B cells (e, FAP negative but HLA-A*02:01, NY-ESO-1157-165 peptide positive) at different effector to target ratios and analyzed after 4 h of incubation in a europium release assay (n = 2). Error bars were calculated as mean + SD.