Figure 4

Cell recovery and viability of moDCs and LCs after electroporation and cryopreservation. Immature (□) and partially-matured (▤) moDCs and LCs were electroporated with eGFP-encoding mRNA, without purification by HLA-DR selection a priori. (A) Immediate post-electroporation viable cell recovery relative to the total number of cells electroporated was assessed by trypan blue exclusion (mean ± SD, n = 4 independent experiments). (B) Surviving cells from (A) were returned to culture, and cell viability was assessed 24 hours after electroporation, relative to initial number of cells returned to culture after electroporation (mean ± SD, n = 4 independent experiments). (C) After at least 24 hours in culture, cells from (B) were cryopreserved. Upon subsequent thawing, immediate post-thaw viability was assessed relative to initial number of cells frozen (mean ± SD, n = 4 independent experiments). (D) After 24 hours in culture, thawed cells from (C) were reassessed for viability (mean ± SD, n = 4 independent experiments).