Figure 2

Optimization of mRNA-electroporation conditions. Immature moDCs (A-C) and partially-matured LCs (D-F) were electroporated with eGFP-encoding mRNA under different conditions of set voltage, number of electroporation pulses, or amount of mRNA. Only one of the three parameters was varied in any given set of tested conditions, as summarized in each panel. Cells were assessed for viability (○) by trypan blue exclusion and transfection efficiency (▤) by flow cytometry.