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Figure 2 | Journal of Translational Medicine

Figure 2

From: Langerhans-type and monocyte-derived human dendritic cells have different susceptibilities to mRNA electroporation with distinct effects on maturation and activation: implications for immunogenicity in dendritic cell-based immunotherapy

Figure 2

Optimization of mRNA-electroporation conditions. Immature moDCs (A-C) and partially-matured LCs (D-F) were electroporated with eGFP-encoding mRNA under different conditions of set voltage, number of electroporation pulses, or amount of mRNA. Only one of the three parameters was varied in any given set of tested conditions, as summarized in each panel. Cells were assessed for viability () by trypan blue exclusion and transfection efficiency () by flow cytometry.

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