Demonstrates MUC20 mRNA expression and cell migration in vitro (Envision × 40). (A) Relative expression of MUC20 in CRC tissues and ANCT. The expression of MUC20 was quantified by qRT-PCR and normalized to GAPDH. (B) Relative expression of MUC20 in CRC cell lines. (C-F) Representative images of the wound healing assay with LoVo transfected with vector and shRNA. Cell migration was photographed and assessed by measuring gap sizes (inserted number represented percentage area of gap ± SD). (G-J) Representative images of the wound healing assay with SW620 transfected with vector and pIRES2-EGFP-MUC20. Data represent mean ± SD of triplicates. *: P < 0.05 in a comparison of the shRNA or pIRES2-EGFP-MUC20 treated group with the mock vector groups.