Figure 1

High (panel A) and low (panel B) microvessel area (MVA) in matched primary and metastatic specimens by AQUA. We used a cocktail of anti-cytokeratin and anti-carbonic anhydrase-9 conjugated to Cy2 to create a tumor mask (green), and anti-CD-34 conjugated to Cy5 (red) to identify microvessels. An example of a patient with high MVAs in both the primary and metastatic specimens is shown in panel A, and an example of low MVAs in both primary and metastatic specimens is shown in panel B. The corresponding MVA scores were 14.92% and 18.93%, respectively for panel A and 4.26% and 6.78%, respectively for the patient represented in panel B.