Complementary serum proteomic analysis of autoimmune hepatitis in mice and patients

Table 2 The peptides of C3 and A2M were identified by iTRAQ analysis

Protein name	Peptide sequence	Ion score^a	Peptide ratio (116/114)^b	Protein ratio (116/114)^c
alpha-2-macroglobulin	AIGYLNTGYQR.	43	1.708	1.61 ± 0.14
	GVPIPNK.	19	1.496
	SASNMAIVDVK.	75	1.727
	SIYKPGQTVK.	48	1.401
	ALLAYAFALAGNQDKR.	77	1.499
complement C3^d	LVAYYTLIGASGQR	77	2.9	2.19 ± 0.78
	AGDFLEANYMNLQR	48	1.363
	YFKPGMPFDLMVFVTNPDGSPAYR	45	1.385
	DQLTCNK	42	1.316
	TGLQEVEVK	54	1.932

^aProbability-based Mascot scores.
^bThe peptides of C3 and A2M were identified by iTRAQ analysis (Control: 114; AIH: 116).
^cThe differential expression of C3 and A2M in serum between AIH patients and controls was calculated based on the isotope labeling peptides.
^d Peptide “⁵³¹LVAYYTLIGASGQR⁵⁴⁴” is a part of C3β chain.
Peptide “¹¹⁷²AGDFLEANYMNLQR¹¹⁸⁵” is a part of C3d fragment.
Peptide “³⁶³YFKPGMPFDLMVFVTNPDGSPAYR³⁸⁶” is a part of C3β chain.
Peptide “¹³⁵⁴DQLTCNK¹³⁶⁰” is a part of C3c α chain fragment 2.
Peptide “⁹⁰⁵TGLQEVEVK⁹¹³” is a part of C3c α chain fragment 1.

ISSN: 1479-5876