Table 1 Sequences of primers used in qRT-PCR

Acetyl-CoACarboxylase Alpha (ACACA)

5′-CCCAGATTCTGCGTT TAAGA -3′

Long-chain fatty acid synthesis

5′-CATCCACATGT AAGCACCA -3′

Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1)

5′-TACATTCGCTTTCTGCAACA -3′

Transcriptional activator for lipid homeostasis

5′- GTCAGGTTCC TCCACCTC -3′

Peroxisome Proliferator-Activated Receptor Gamma (PPARG)

5′-CCC AAG TTT GAG TTT GCT GT -3′

Transcription factor

5′- AAC AGC TGT GAG GAC TCA GG3′

Glycogen Synthase Kinase 3 Beta interaction protein ninein (NIN)

5′- GAA TGT GGA TGG AGA GAT GC

Glycogen metabolism

5′- ATT GCT GAT GAG GTT GTG GT 3′

RAF proto-oncogene serine/threonine-protein kinase (RAF1)

5′- ATCCGA ATGCAG GAT AAC AA 3′

Regulator inCell fate decisions

5′- AAG ATC TGG GGA GGC ATA TC3′

Fructose-1, 6-Bisphosphatase 1 (FBP1)

5′- TCA ACT GCT TCA TGC TGG AC -3′

Gluconeogenesis regulator

5′-CGT AGACCA GAG TGC GAT GA3′

Acyl-CoA Oxidase 1 (ACOX1)

5′-CTG AAG GCT TTC ACC TCC TG -3′

Fatty acid Beta-Oxidation pathway

5′-CAT GCC ACACACCAACTT TC -3′

Low Density Lipoprotein Receptor (LDLR)

5′-CCA AACCCC TAA ACTCAG GA 3′

Receptor-mediated endocytosis of LDL

5′- AAG TGGCATCAT TTG GTG AA 3′

Sorbin and SH3 DomainContaining 1 (SORBS1)

5′-CTGCAAGCCCACAGTTTTCCAGT-3′

Insulin-stimulated glucose transport

5′-CGAGCAGCTTTCCTCCCCGC-3′

Protein phosphatase 1 (PPP1CA)

5′- ACC TGC AGTCTA TGG AGC AG 3′

Glycogen metabolism

5′- TAGCCG TCT TCT ACC ACC TG -3′