A proteomic view of isoproterenol induced cardiac hypertrophy: Prohibitin identified as a potential biomarker in rats

Table 1 List of primer sequences for gene of interest

Gene of interest	Primers	Sequence	T_m
MYL2	Forward primer	5'-GAAAGCCAAGAAGAGGTTAGAGG-3'	55°C
MYL2	Reverse primer	5'-ATGGTGAGGAACACAGTGAAGTT-3'	55°C
MYL3	Forward primer	5'-AAGAGCTCAACTCCAAGATGATG-3'	55°C
MYL3	Reverse primer	5'-TCTCTACCTCGTCTTCTGTCAGC-3'	55°C
PEBP1	Forward primer	5'-GTCATGAATAGACCAAGCAGCAT-3'	57°C
PEBP1	Reverse primer	5'-CTCATACACCAGCCAGACGTAG-3'	57°C
PHB	Forward primer	5'-GGGTACAGAAGCCAATCATCTTT-3'	55°C
PHB	Reverse primer	5'-AATGCTGGTGTAGATACGAGGAA-3'	55°C
HSP60	Forward primer	5'-GCAGAGTTCCTCAGAGGTTGG-3'	53°C
HSP60	Reverse primer	5'-CCCAGCAGCATCCAGTAAAG-3'	53°C
Atp5b	Forward primer	5'-TGTATTTGCTGGTGTTGGTGA-3'	53°C
Atp5b	Reverse primer	5'-ACCTTGGAAGTGGCATCTTTT-3'	53°C
Desmin	Forward primer	5'-CTGATAGACGACCTGCAGAGG-3'	55°C
Desmin	Reverse primer	5'-AAGGAATGCAATCTCCTCGTT-3'	55°C
GAPDH	Forward primer	5'-TGGTGCTCAGTGTAGCCCAG-3'	58°C
	Reverse primer	5'-GGACCTGACCTGCCGTCTAG-3'

ISSN: 1479-5876