Table 1 Primer sequences of various genes
Genes | Primer sequences | |
|---|---|---|
Insulin 1 | Forward | 5’-CAGTTGGTAGAGGGAGCAG-3’ |
Reverse | 5’-CAGTTGGTAGAGGGAGCAG-3’ | |
Insulin 2 | Forward | 5’-TCATCCTCTGGGAGCCCCGC-3’ |
Reverse | 5’-AGTTGCAGTAGTTCTCCAGT-3’ | |
Ngn3 | Forward | 5’-CTTCACAAGAAGTCTGAGAACACCAG-3’ |
Reverse | 5’-CTGCGCATAGCGGACCACAGCTTC-3’ | |
Pdx-1 | Forward | 5’-GGTGCCAGAGTTCAGTGCTAA-3’ |
Reverse | 5’-CCAGTCTCGGTTCCATTCG-3’ | |
Nkx 6.1 | Forward | 5’-ATGGGAAGAGAAAACACACCAGAC-3’ |
Reverse | 5’-TAATCGTCGTCGTCCTCCTCGTTC-3’ | |
Nkx 6.2 | Forward | 5’-AGAAAGGTATGGAGGTGACG-3’ |
Reverse | 5’-CTGTACTGGGCGTTGTATTG-3’ | |
β-Actin | Forward | 5’-GCTGTGTTGTCCCTGTATGC-3’ |
Reverse | 5’-GAGCGCGTAACCCTCATAGA-3’ | |