Figure 4

PEP-1-CAT regulated Bcl-2 and Bax expression. (A) Bcl2 and Bax mRNA expression was detected by qRT-PCR and normalized to β-actin. ^*P<0.01 vs control (CTL); ^&P<0.05 vs H/R; ^#P<0.05 vs H/R or H/R + CAT (CAT); n = 3. (B) Bcl2 and Bax protein expression was detected by western blot. (C) Normalization of Bcl2 and Bax expression to α-Tubulin. ^*P<0.01 vs control (CTL); ^&P<0.05 vs H/R; ^#P<0.05 vs. H/R or H/R + CAT (CAT); n = 4. (D) Bcl2/Bax ratio. ^*P<0.01 vs control (CTL); ^&P<0.05 vs H/R; ^#P<0.05 vs H/R or H/R + CAT (CAT); n = 4.