Figure 3

PEP-1-CAT inhibited H/R-induced H9c2 cell apoptosis. H9c2 cells were pretreated with CAT or PEP-1-CAT for 6 h and placed into normoxia environment for 27 h or into hypoxia chamber for 21 h followed by 6 h reoxygenation. (A) Cell apoptosis was detected by DAPI staining. (B-C) H/R-induced H9c2 apoptosis rate was quantiied by Flow Cytometry. *P<0.01 vs control (CTL);^&P<0.05 vs H/R; ^#P<0.01 vs H/R or H/R + CAT (CAT); n = 5. (D) PARP and caspase-3 protein expression was detected by western blot.