Figure 5

Reconstitution of SATB1 expression in LNCaP cells. LNCaP cells were untransfected (control), transfected with pcDNA3.1 control vector or transfected with pcDNA3.1-SATB1 vector for 12 h, 24 h, or 36 h. A. The mRNA level of SATB1 was detected by RT-PCR (n = 3). B. The protein level of SATB1 was detected by Western blot analysis (n = 3). β-actin served as loading control. *P < 0.05 compared to cells transfected with pcDNA3.1.