Figure 3

Silencing of SATB1 in DU-145 cells. DU-145 cells were untransfected (control), transfected with pSliencer3.1 control vector or transfected with pSilencer3.1-SATB1 vector for 24 h, 48 h, or 72 h. The protein levels of SATB1 and SATB2 were detected by Western blot analysis (n = 3). β-actin served as loading control. * P < 0.05 compared to cells transfected with pSliencer3.1.