Figure 1
Generation of subcutaneous PC14PE6-RFP lung adenocarcinomas and tumor-associated ME. (A) Photographic image of a mouse bearing a subcutaneous PC14PE6-RFP tumor on the right flank 14 dpim showing a hematoma around the tumor site and the beginning of exudate accumulation in the groin (arrowhead). (B-C) exemplary T1w MRI of a PC14PE6-RFP-bearing mouse 22 dpim revealing tumor-associated effusion in the groin containing fluid (black asterisks, B) and solid/semi-solid cellular material (white asterisk, B). Using T1w MRI the time-dependent distribution of (iv) injected Gd-DTPA was recorded in different tumor regions and areas of the ME as indicated by regions of interest (ROIs) in (C); the corresponding SNR over time of the different ROIs were plotted in (D). ROI coloring: red – necrotic tumor region, blue – enlarged blood vessel, yellow – non-necrotic tumor region, green – outer effusion region, pink – solid effusion content, light blue – fluid effusion content, black – control muscle tissue. (E) micro-photographic image overlayed with the red fluorescent image of an exudate smear revealing numerous erythrocytes as well as red-fluorescent tumor cells and non-fluorescent cells. (F) FACS analysis of PC14PE6-RFP tumors (28 dpim, n = 4) and ME (n = 3); shown are the percentage of RFP-positive tumor cells, propidium iodide (PI)/RFP-positive dead tumor cells, and CD45-positive immune cells in tumors and ME.