Figure 2
Effect of PsL-EGFmAb on renal DC maturation. A-C, Renal DCs from control (A), NTN (B) and PsL-EGFmAb (C) groups were freshly isolated by MACS using anti-rat OX62 micro-beads on day 14 and maturity of DCs was determined by flow cytometry detected by cell surface expression of MHC class-II, CD80 and DC-SIGN (black line). The isotype-matched controls are shown as gray line. D, Allogeneic mixed lymphocyte reactions were used to evaluate the proliferation of CD4+ T cells induced by isolated DCs. MACS-isolated and irradiated DCs from rat kidneys of control, NTN and PsL-EGFmAb-treated groups were co-cultured with CD4+ T cells from healthy rats at a 1:10 ratio for five days. T cell proliferation was assessed by [3H]TdR incorporation. The results were shown as fold increases in [3H]TdR incorporation. The mean ± SD of three independent experiments is shown. **p < 0.01 vs. control; ##p < 0.01 vs. NTN group.