Volume 10 Supplement 2

Proceedings of the 2012 Sino-American Symposium on Clinical and Translational Medicine (SAS-CTM)

Open Access

Evaluation of cardiac function at different time points after myocardial infarction of rats

  • Huiyuan Hu1,
  • Yan Zhao1,
  • Shuang Liu2,
  • Wang Yan1,
  • Aosiman Yushupujiang1,
  • Sitong Jin1,
  • Xuefei Sun1,
  • Weidong Ren2 and
  • Liying Hao1Email author
Journal of Translational Medicine201210(Suppl 2):A58

https://doi.org/10.1186/1479-5876-10-S2-A58

Published: 17 October 2012

Backgrounds

Myocardial infarction (MI) model of rats is commonly used in cardiovascular research. However, the noninvasive functional evaluation parameters in this model are lack. Echocardiography is an important tool for assessment of cardiac function in clinic. This study is to explore whether changes of the cardiac function after MI of rats could be detected by echocardiography accurately.

Methods

MI were induced in rats by ligating the left anterior descending coronary artery. Cardiac function and pathological changes were measured by Dimension echocardiography and transmission electron microscope (TEM) at 24h, 1w, 2w and 4w after MI, respectively.

Results

Compared with sham group, cardiac function did not change in 24h group after ligation, but the interventricular septum thickness at end-systole and interventricular septum thickness at end-diastole in groups of 1, 2, 4 weeks after operation were significantly decreased (P<0.05); the left ventricular internal dimension at end-systole in groups of 1, 4 weeks after MI and left ventricular internal dimension at end-diastole in 4w group were dramastically increased (P<0.05); the ejection fraction and left ventricular fraction shortening in groups of 1, 2, 4 weeks after MI declined significantly (P<0.05); in addition, the mitral valve E peak in 2w group was greatly reduced (P<0.05). These data suggested that both left ventricular systolic and diastolic function were affected 1 week after MI, with changes of cardiac sturcture. Moreover, TEM illustration showed that nuclear pyknosis, myofilament disruption, mitochondrial swelling were present in all MI groups and severe reconstruction and fibrosis were observed especially in 4w-MI group.

Conclusion

Although cardiac structure was damaged 1 day after MI, its funciton was unchaged due to compansatory effect of normal tissues. With time goes on, the infarcted cardiac tissue was severely deformed and cardiac function was decreased accordingly. Ecochardiography could detect the changes of cardiac funciton accurately after MI, which will provide important experimental evidences for the clinical diagnosis, treatments and prognostic assements of MI patients.

Declarations

Acknowledgement

This work was supported by Natural Science Foundation of China Grants (81100108, 31071004) and scientific research project of Education Department of Liaoning Provice (L2010572).

Authors’ Affiliations

(1)
Dept. of Pharmaceutical Toxicology, China Medical Univ
(2)
Dept. of Cardiovascular funciton, China Medical Univ

Copyright

© Hu et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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