Table 3 The primer sequences and restriction enzymes used for detection of cytokine DNA polymorphism genes
Cytokines | Primers | Sequence | Position | Restriction Enzyme | Digestion Time (hours) | References |
|---|---|---|---|---|---|---|
IL-1α | Forward | 5'ATGGTTTTAGAAATCATCAAGCCTAGGCA-3' | -889 | Fnu 4H1 | >12 | Walker et al. |
| Â | Reverse | 5'AATGAAAGGAGGGGAGGATGACAGAAATGA-3' | Â | Â | Â | Â |
IL-1β | Forward | 5'-TGGCATTGATCTGGTTCATC-3' | -511 | Aval | >12 | Néstor et al. |
| Â | Reverse | 5'-GTTTAGGAATCTTCCCACTT-3' | Â | Â | Â | Â |
IL-6 | Forward | 5'TTGTCAAGACATGCCAAGTGCT-3' | -174 | Nla III | 4 | Trevilatto et al. |
| Â | Reverse | 5'-GCCTCAGAGACATCTCCAGTCC-3' | Â | Â | Â | Â |
TNF-α | Forward | 5'-GAAGCCCCTCCCAGTTCTAGT TC-3' | -238 | Ava II | 4 | Sleijffers et al. |
| Â | Reverse | 5'-CACTCCCCATCCTCCCTGGTC-3' | Â | Â | Â | Â |
IFN-γ | Forward | 5'-GCTGTCATAATAATATTCAGAC-3' | -874 | Alw | 4 | Inoue et al |
| Â | Reverse | 5'-CGAGCTTTAAAAGATAGTTCC-3' | Â | Â | Â | Â |
IL-4 | Forward | 5'-TAAACTTGGGAGAACATGGT-3' | -590 | Ava II | >12 | Scarel-Caminaga et al. |
| Â | Reverse | 5'-TGGGGAAAGATAGAGTAATA-3' | Â | Â | Â | Â |
IL-10 | Forward | 5'-CTCGCTGCAACCCAACTGGC-3' | -1082 | Mnl I | 4 | Chin et al. |
| Â | Reverse | 5'-TCTTACCTATCCCTACTTCC-3' | Â | Â | Â | Â |