Table 1 Replacement of R&D starting materials to GMP compliant starting materials

Enzymes

Dispase II (Roche Diagnostics, Mannheim, Germany)

Collagenase NB6 (Serva, Heidelberg, Germany)

Serum

Fetal calf serum (Biochrom AG, Berlin, Germany)

Human serum (pooled AB serum, Centre for Clinical Transfusion Medicine Tuebingen, Germany)

Supplements

Penicillin/Steptomycin (Invitrogen, Carlsbad, USA) Heparin (Sigma, St. Louis, USA) Basic fibroblast growth factor (Sigma, St. Louis, USA)

Gentamicin (PAA, Pasching, Austria) Heparin (pharmaceutical grade, Ratiopharm, Ulm, Germany) Basic fibroblast growth factor (clinical grade, Peprotech, Hamburg, Germany)

Cryopreservation

Fetal calf serum (Biochrom AG, Berlin, Germany) Dimethyl sulfoxide (Sigma, St. Louis, USA)

Human serum albumin (20 %, pharmaceutical grade, Baxter, Unterschleißheim, Germany) Dimethyl sulfoxide (pharmaceutical grade, Charité- Universitätsmedizin Berlin, Germany, product of the dispensary)