Figure 7

Differentiation potential of human umbilical cord artery derived cells (HUCAC) compared with mesenchymal stem cells (MSC). Multilineage differentiation potential of HUCAC from passage 2 (n = 3), passage 4 (n = 3) and MSC (positive control) from passage 2 (n = 1), passage 4 (n = 1) was qualitatively analyzed by histological and immunohistochemical staining methods. Osteogenic differentiation potential was studied by histological Von Kossa staining: A) unstimulated HUCAC (day 28), B) stimulated HUCAC (day 28), C) stimulated MSC (day 28) and histological alkaline phosphatase staining: D) unstimulated HUCAC (day 28), E) stimulated HUCAC (day 28), F) stimulated MSC (day 28). Adipogenic differentiation potential was studied by histological Oil Red O staining: G) unstimulated HUCAC (day 25), H) stimulated HUCAC (day 25), I) stimulated MSC (day 25). Chondrogenic differentiation potential was studied by histological Alcian PAS staining: J) unstimulated HUCAC (day 28), K) stimulated HUCAC (day 28), L) stimulated MSC (day 28) and by immunohistochemical collagen type II staining: M) unstimulated HUCAC (day 28), N) stimulated HUCAC (day 28), O) stimulated MSC (day 28).