Figure 4

Intraperitoneal administration of 3PO suppresses delayed type hypersensitivity to mBSA. (A), Six BALB/c mice were injected subcutaneously with 250 μg mBSA emulsified with CFA and, seven days following immunization, the mice were challenged with an injection of 50 μl of 0.5 mg/ml mBSA in saline in one rear foot pad and 50 μl saline in the other rear footpad and then administered either vehicle (DMSO) or 3PO (0.07 mg/gm) 30 minutes and 12 hrs after the challenge. Footpad thickness was measured 24 hours following mBSA challenge using microcalipers. After the mice were euthanized, the draining popliteal lymph nodes were removed and measured with microcalipers (B) and the total number of lymphocytes counted using light microscopy (C). Data are representative of three independent experiments. *p < 0.05 (relative to vehicle control).