Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE® bioreactor

Table 1 A table of IFN-γ secretion in response to HLA matched and mismatched cell lines and to T2 cell pulsed with peptide recognized by the transduced TCR

	Melanoma Cell Lines				T2 cells pulsed with peptide (μM/ml)
			938	624	Irrelevant Peptide		Specific Peptide
	TCR	Bioreactor	A2-	A2+	1.0	1.0	0.1	0.01	0.001	0.0001
NY-ESO	Patient 1	Static Bags	40	19300	44	> 126650	45600	5095	1080	136
		Wave	68	46950	128	> 182100	83200	23350	2770	646
	Patient 2	Static Bags	345	39700	694	> 241000	52250	4195	985	339
		Wave	410	40700	1876	> 219650	58400	8650	2374	1302
	Patient 3	Static Bags	79	> 31250	84	> 84800	23850	2425	242	40
		Wave	78	> 30150	120	> 83000	31500	4830	534	139
	Patient 4	Static Bags	16	4725	27	11045	1075	166	45	29
		Wave	18	5770	43	12445	1610	236	54	33
	Patient 5	Static Bags	7	11780	855	> 113300	25555	2635	1346	14
		Wave	1201	16140	147	> 125300	63400	8230	469	177
	Patient 6	Static Bags	129	3505	105	9535	1080	147	40	12
		Wave	36	4000	99	26950	2560	315	118	80
	Patient 7	Static Bags	23	6565	40	24750	3445	818	393	159
		Wave	78	3550	290	> 45100	6240	911	441	204
MART-1	Patient 8	Static Bags	98	> 77225	160	> 58200	11480	1375	544	NA
		Wave	148	91625	547	62200	20875	3630	1510	NA
	Patient 9	Static Bags	12	66500	30	> 180600	93700	370	490	NA
		Wave	65	> 162700	197	> 260700	> 154600	3000	2897	NA
gp154	Patient 10	Static Bags	18	32950	22	NA	4360	2600	739	479
		Wave	18	42650	55	NA	8075	5290	1785	684

ISSN: 1479-5876