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Table 1 The prepared culture media

From: Responses of human adipose-derived mesenchymal stem cells to chemical microenvironment of the intervertebral disc

 

DMEM (4.5 mg/mL)

DMEM (1.0 mg/mL)

FCS (%)

Penicillin (U/mL)

Streptoymcin (μg/mL)

5 M NaCl 0.4 M KCl (%)

pH

characteristics

Standard medium

√

 

10

50

50

 

7.4

4.5 mg/mL glucose

280 mOsm pH 7.4

IVD-like glucose

medium

 

√

10

50

50

 

7.4

1.0 mg/mL glucose

280 mOsm pH 7.4

IVD-like osmolarity

medium

√

 

10

50

50

2

7.4

4.5 mg/mL glucose

485 mOsm pH 7.4

IVD-like pH

medium

√

 

10

50

50

 

6.8

4.5 mg/mL glucose

280 mOsm pH 6.8

IVD-like combined

medium

 

√

10

50

50

2

6.8

1.0 mg/mL glucose

485 mOsm pH 6.8