Figure 2

The viability of ADMSCs under IVD-like chemical conditions. The numbers of viable, apoptotic, and secondary necrotic cells were determined by annexin V-FITC and PI (AV-PI) staining. Compared to the standard condition, IVD-like glucose condition slightly decreased the number of viable cells while IVD-like osmolarity, IVD-like pH and combined conditions significant decreased the number of viable cells and increased the number of apoptotic cells.